No antibody or IFN-gamma responses were detected in the control group immunized with an empty plasmid or not immunized. Aqualysin I is a heat-stable subtilisin-type serine protease which is secreted into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. The cytotoxicity tests based on the measurement of lactate dehydrogenase LDH activity were performed using a fibroblast HS-5 cell line. Nutrients ; 11 9 Aug The lipids of human milk after holder pasteurization had the lowest content of free fatty acids and the shortest induction time compared with samples after HPP. In the group immunized with Emulsigen P as an adjuvant, lower IgG1 and IgG2 antibody levels were developed compared to the other treatment groups. The aim of this research was to evaluate the immune responses of sheep injected twice, intramuscularly, with DNA plasmids encoding T. Mol Immunol ; 44 5 :Feb. It was proposed that this method could be used as an alternative to holder pasteurization HoP; These findings suggest that current omega-3 FA intake does not translate directly into their concentration in human milk.
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The correlation coefficient Pearson's r did not show a significant statistical relationship between human milk composition and nutrients in women's diet at three time points.
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Ann Agric Environ Med ; 26 1 :Mar Comparison of immune response in sheep immunized with DNA vaccine encoding Toxoplasma gondii GRA7 antigen in different adjuvant formulations. Protein Expr Purif ; 29 2 :Jun. Milk samples were obtained from women during weeks of lactation.
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|The results demonstrate that the intramuscular injection of sheep with a DNA liposome formulated plasmid coding for GRA proteins is an effective system that induces a significant immune response against T.
After the first inoculation, moderate to high antibody responses were observed with the three different adjuvant formulations. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease - Acanthamoeba keratitis, mostly among contact lens wearers.
Post-treatment culture showed no endogenous bacterial contamination in any tested option. The aim of this research is to evaluate the immune responses of sheep immunized with DNA plasmids encoding Toxoplasma gondii dense granule antigen GRA7 formulated into three different adjuvant formulations. High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris.
To compare the effects of high pressure processing HPP and holder pasteurization on lipidome, human milk was processed at
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Instite of High ressure Physics Polish Academy. Aneta Czerwonogrodzka-Senczyna · Edyta Jasińska-Melon; [ ] Dorota Szostak- Sep Sylwester J Rzoska · E. Rosiak · Malgorzata Rutkowska; [ ].
The oxidative induction time was obtained from the pressure differential scanning calorimetry.
This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. In the group immunized with Emulsigen P as an adjuvant, lower IgG1 and IgG2 antibody levels were developed compared to the other treatment groups.
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Kendall's rank correlation coefficients were 0. This study determined fatty acid FA concentrations in maternal milk and investigated the association between omega-3 fatty acid levels and their maternal current dietary intake based on three-day dietary records and habitual dietary intake based on intake frequency of food products.
The expression of aqualysin I in P. The level of all analyzed components were significantly decreased by HoP: leptin